MAKING_LIFE 2014 - 2015

Protocells - talk
posted by sarah on 29 March 2016

After the hands-on session we continued with a lecture by Martin Hanczyc who’s a Principal Investigator in the Centre for Integrative Biology (CIBIO) at the University of Trento, Italy. The research of the Hanczyc Lab is focused on understanding the fundamental principles of living and evolving systems through experimental science. To this end, he builds synthetic systems where dynamic life-like properties emerge in simple chemical experiments.

  “Like other sciences, synthetic biology must proceed from the simpler to the more complex, beginning with the reproduction of the more elementary vital phenomena.”
– Stéphane Leduc, The Mechanisms of Life, 1911

“Protocells” can be considered a bottom-up approach to synthetic biology. The term first emerged in the context of research on the origins of life and artificial life research in the 1930’s. Martin defines protocells as “a simple artificial prototype of a living cell with one or more properties of life”.

In general, there can be different possible forms of containment and colocalization: liposomes, polymersomes, bubbles, droplets, crystals, coacervates (jelly-like substance that diffuses very slowly), standing waves …
Martin mainly focuses on dynamic droplet systems. The first experimental setup seems to be very simple – it’s just a dish of olive oil and droplets of sodium hydroxide (NaOH) solution with some food coloring. This setup can be considered a typical water-and-oil system or so-called “Bütschli” system. Otto Bütschli first described the system in 1898, when he used alkaline water droplets in olive oil to initiate a saponification reaction. We can see the droplets deform and slowly break up.

In the microscopic view we can even see some life-like behaviour emerging from the chemical-physical phenomena.

The second dynamic droplet system Martin presents is about self-movement and related pH gradients: A motile droplet (red) is placed into an aqueous solution containing a pH indicator. When the pH of the solution is above pH 11, the solution is blue. When the solution becomes acidic, the solution loses its color. This is the “recipe” and how it looks:

5ul nitrobenzene and oleic anhydride droplet
0.8ml water pH 12.0
0.1mg/ml pH indicator Thymolphthalein


The point here is to create a situation which is far away from a state of equilibrium. The difference between the pH value of the droplet and the pH value of the surrounding solution is high at the beginning and becomes lower and lower until equilibrium is reached and the droplet stops moving. (The droplet’s internal “metabolism” is actually an exothermic reaction in which energy is released.)

Another experiment is about droplet chemotaxis. It’s basically the same experiment as before, just that additionally, a drop of NaOH solution is added to the experiment. The droplet becomes some kind of “pH sensor”, which “reacts” to its environment depending on the different pH levels. The droplet senses the NaOH solution, approaches it and even “climbs” to the point of highest concentration. This can be actually compared to biological chemotaxis, for example when bacteria move to an area where food, e.g. glucose has been added.  
5ul nitrobenzene and oleic anhydride droplet
0.8ml water pH 12.0
0.1mg/ml pH indicator
0.1ul 3M NaOH gradient


Competing flows and movements within the protocell can sometimes even lead to cell division, a probabilistic event that sometimes happens and sometimes doesn’t.

To summarize, droplets are dynamic when loaded with chemical potential (energy) and far from equilibrium. Life-like behaviors emerge such as self-movement, chemotaxis as well as rudimentary replication cycles.

Talking about the origins of life in relation to protocells, Martin references to the well-known Miller-Urey experiment from 1953, in which a diverse mixture of organic compounds emerges from inorganic starting materials, generating some kind of “primordial ooze” which is too difficult to fully characterize. So instead of using pure chemicals, Martin also made some experiments in which he uses tar-like material to create protocells. To be more precise, he uses HCN polymers which were developed similarly to the sludge produced in the Miller-Urey experiment. The protocells created from that “messy” material are also able to move around and to sense their environment. This refers to a hypothesis about the origin of life called “maximalism“. Instead of assuming that life started simple and became more and more complex (monomers > polymers > self-replicating structures > evolving cells), the maximalism hypothesis states that in the beginning, there was a complex tar-like substance from which the less complex monomers developed in the first place.

The last video shows droplets created from HCN polymers made in Martin’s lab which demonstrate movement and chemotaxis.